Autonomous En/Spm DNA transposon from zebrafish
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EnSpm-2_DR is young family of autonomous En/Spm DNA transposons. The consensus sequence was derived based on multiple alignment of several copies of EnSpm-2_DR that are less then 5% divergent from each other. The consensus sequence codes for a 815-aa En/Spm transposase. Based on multiple alignment of diverse En/Spm transposases from plants, fungi and animals, we suggest that the En/Spm catalytic core is defined by a DDE triad. Its coordinates in EnSpm-2_DR transposase are defined by the 380-469-634 aa positions. Zebrafish EnSpm transposase contains also in its N-terminal portion a novel type of zinc finger motif, called ENS-finger: C-x2-C-x12-H-x4-H-x11-C-x4-C-x12-H-x4-H. EnSpm-2_DR transposons are characterized by 2-bp target-site duplications (TA - most frequent, CA, TG) and imperfect 30-bp terminal inverted repeats (6 mismatches). EnSpm-2_DR is a composite transposon: its 869-1079 portion is similar to the 172-388 portion of the TDR15 transposon. Contrary to plant En/Spm transposons with the canonical CACTA 5'-termini, zebrafish En/Spm transposons contain the CACAG or CACTG 5'-termini. Zebrafish En/Spm transposons differ also from their plant relatives by a target-site duplication length: it is 2 bp in zebrafish (3 bp in plants).
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Number of matches to this model that meet the "Gathering" threshold and more stringent "Trusted" threshold. For each threshold, two numbers are shown: (1) the number of matches to this model after removing redundant hits to other models ("non-redundant"), and (2) the total number of matches above threshold, including those with better scoring matches to other models ("all hits").
The model is 7988 positions long. The average length of non-redundant hits to the model is 392.1. This table shows the number of hits above score thresholds:
|non-redundant||all hits||non-redundant||all hits|
External Database Links
- Repbase : EnSpm-2_DR [Requires Repbase registration]