EnSpm-2_DR (DF0003911)

Autonomous En/Spm DNA transposon from zebrafish

Description

EnSpm-2_DR is young family of autonomous En/Spm DNA transposons. The consensus sequence was derived based on multiple alignment of several copies of EnSpm-2_DR that are less then 5% divergent from each other. The consensus sequence codes for a 815-aa En/Spm transposase. Based on multiple alignment of diverse En/Spm transposases from plants, fungi and animals, we suggest that the En/Spm catalytic core is defined by a DDE triad. Its coordinates in EnSpm-2_DR transposase are defined by the 380-469-634 aa positions. Zebrafish EnSpm transposase contains also in its N-terminal portion a novel type of zinc finger motif, called ENS-finger: C-x2-C-x12-H-x4-H-x11-C-x4-C-x12-H-x4-H. EnSpm-2_DR transposons are characterized by 2-bp target-site duplications (TA - most frequent, CA, TG) and imperfect 30-bp terminal inverted repeats (6 mismatches). EnSpm-2_DR is a composite transposon: its 869-1079 portion is similar to the 172-388 portion of the TDR15 transposon. Contrary to plant En/Spm transposons with the canonical CACTA 5'-termini, zebrafish En/Spm transposons contain the CACAG or CACTG 5'-termini. Zebrafish En/Spm transposons differ also from their plant relatives by a target-site duplication length: it is 2 bp in zebrafish (3 bp in plants).

Classification

Accession Name Wikipedia
Type DNA Transposon Article
Class Cut and Paste
Superfamily CMC-EnSpm

Hit Statistics

The model is 7988 positions long. The average length of non-redundant hits to the model is 392.1. This table shows the number of hits above score thresholds:

Species Gathering Trusted
non-redundant all hits non-redundant all hits
Danio rerio 458 15703 351 11475

External Database Links

  • Repbase : EnSpm-2_DR [Requires Repbase registration]